MICROBIAL BIOTECHNOLOGY COMBO KIT (4 WORK STATIONS)

AIM

To extract and visualize DNA from an intestinal bacteria in exponential growth phase.

To produce two different type of dairy products using microorganisms (acid coagulation for the production of yogurt and enzymatic coagulation for the production of junket) , to analyse the resulting product s and their final pH and observe the the bacteria that the yogurt contains.

To product dextrin using microorganisms.

To analyse the capacity of certain microorganisms to produce enzymes that can be useful in certain areas of biotechnology, such as biodetergent production, by doing a qualitative analysis of the amylase and protease enzymes.

LEVEL

Can be adapted to O-level, A/AS-level, Science colleges and Universities.

TECHNIQUES

Inoculation and incubation of microorganisms in the culture media that is specific for them to grow.

Extraction and visualization of DNA processes.

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DURATION

Practice sessions can be overlapped for time saving. Please, check the duration of each individual practice.

CONTENT

Each kit contains four work stations with all the necessary material and components to perform the experiments: racks, microorganisms, culture media, lysis solution and precipitation solution, material for microbial inoculation, staining solution, material for measuring the pH and reactive material to detect enzymes production.

The kit also contains an interactive CD-Rom:
- Instructions of use
- Aims
- Organization
- Background
- Conceptual maps
- Protocols
- Supplementary activities
- Aseptic techniques& safety rules
- Printable versions for the teacher and the students

Suitable for up to 25 students.

ADDITIONAL COMPONENTS REQUIRED

No additional components are required.

REQUIRED EQUIPMENT

Fridge, freezer (optional), culture oven, thermostatic bath (optional), immersion microscope (100X), autoclave.


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