TRANSGENIC DETECTION KIT (4 WORK STATIONS)

AIM

To analyse whether a Genetically Modified Organism (GMO) is maize or soya by studying the zein and lectin genes, respectively.

To do a qualitative analysis of the Cauliflower Mosaic Virus 35S promoter and the Agrobacterium tumefaciens NOS gene.

LEVEL

Can be adapted to O-level, A/AS-level, Science colleges and Universities.

TECHNIQUES

DNA electrophoretic analysis of each sample, which has been previously amplified by PCR.

Staining and visualization of DNA bands.

DURATION

A 50-80 minute practice session plus an additional session for complementary activities.

CONTENT

Each kit contains four work stations with all the necessary material and components to perform the experiments: racks, PCR amplified DNA samples for each of the gene fragments (each group is assigned four genes of a different variety of plants), agarose gels, patented equipment for “easy” loading of samples, electrophoresis and staining solutions.

The kit also contains an interactive CD-Rom:
- Instructions of use
- Aims
- Organization
- Background
- Conceptual maps
- Protocols
- Supplementary activities
- Aseptic techniques& safety rules
- Printable versions for the teacher and the students

Suitable for up to 25 students.

ADDITIONAL COMPONENTS REQUIRED

No additional components are required.

REQUIRED EQUIPMENT

Fridge, electrophoresis gel tank and a 100V power supply unit.


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